Tus txheej txheem ua haujlwm ntawm PCR (polymerase cov tshuaj tiv thaiv) cov khoom lag luam yog raws li cov txheej txheem enzymatic replication txheej txheem ntawm cov txheej txheem DNA.
Cov ntsiab cai ntawm PCC yog siv DNA polymerase rau simulate cov txheej txheem ntawm DNA replication hauv vitro, thiab kom nce tus nqi ntawm dna nthuav dav. Cov kauj ruam tshwj xeeb suav nrog:
DENTURATIONATION: Ua kom sov lub txheej txheem tiv thaiv kom txog 95 degree kom ua tiav cov qauv ua tiav, thiab tib lub sijhawm strand Annaling: Txo qhov ntsuas kub kom haum rau anneal lub primer rau tus DNA. Kev txuas ntxiv: Txhawb qhov kub txog 72 degree, thiab DNA polymerase catalenyzes lub dna synthesis cov tshuaj tiv thaiv nrog DNTP raws li ib substrate. Cov kauj ruam no tsim kev voj voog, thiab cov tshiab synthly DNA molecules txuas ntxiv ua cov qauv rau cov synthesis. Tom qab ntau cov voj voog (feem ntau 20-30 sijhawm), lub hom phiaj ntawm cov tawg disna tuaj yeem ua tiav.